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. Author manuscript; available in PMC: 2016 Jul 15.
Published in final edited form as: J Immunol. 2015 Jun 1;195(2):507–518. doi: 10.4049/jimmunol.1500027

Figure 2.

Figure 2

Ablation of IL-13Rα1 unbalances primary neonatal immunity and skews the responses towards Th1 cells. (A–D) IL-13Rα1+/+ and IL-13Rα1−/− neonatal Balb/c mice recipient of IL-13Rα1−/− or IL-13Rα1+/+ T cells from 1 day old DO11.10 donors were given Ig-OVA or the control Ig-W in saline and 14 days later their splenic primary ex vivo and recall responses were analyzed. (A) Shows a representative ex vivo intracellular IFNγ and IL-4 production by CD4+KJ1-26+ DO11.10 T cells. (B) Shows compiled ex vivo intracellular IFNγ and IL-4 production by CD4+KJ1-26+ DO11.10 T cells from 3 experiments. Each bar represents the mean ± SE. (C) Shows recall IFNγ and IL-4 responses after in vitro stimulation with OVAp as measured by ELISA (top panels) and ELISPOT (bottom panels). Each bar represents the mean ± SD of triplicate wells. (D) Shows compiled results from 5 experiments carried out as described in (C). The bars represent the mean ± SE of the 5 experiments. The statistical analysis used student’s t test to compare results between donor T cells from IL-13Rα1−/− versus IL-13Rα1+/+ mice within both IL-13Rα1−/− and IL-13Rα1+/+ hosts.