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. Author manuscript; available in PMC: 2016 Aug 1.

Published in final edited form as: Mol Oral Microbiol. 2015 Apr 20;30(4):307–322. doi: 10.1111/omi.12095

A: Sixteen-hour mucosal biofilms of C. albicans monospecies (left panel) or C. albicans-streptococci mixed-species (right panel). Biofilms were grown on the surface of three-dimensional models of oral mucosa under wet (media-submerged) conditions. X-Y isosurfaces (top panel) and 3-D reconstructions (bottom panels) of representative confocal laser scanning microscopy images are shown. C. albicans (green) was visualized after staining with a FITC-conjugated anti-Candida antibody. S. oralis (red) was visualized after fluorescence in situ hybridization (FISH) with a Streptococcus-specific probe conjugated to Alexa 546. Scale bar = 50 µm.

B: Sixteen-hour mucosal biofilms of C. albicans monospecies (left panel) or C. albicans-streptococci mixed-species (right panel). Biofilms were grown on the surface of three-dimensional models of oral mucosa under semidry (media limited to inoculum) conditions. X-Y isosurfaces (top panel) and 3-D reconstructions (bottom panels) of representative confocal laser scanning microscopy images are shown. C. albicans (green) was visualized after staining with a FITC-conjugated anti-Candida antibody. S. oralis (red) was visualized after fluorescence in situ hybridization (FISH) with a Streptococcus-specific probe conjugated to Alexa 546. Scale bar = 50 µm.

A: Sixteen-hour mucosal biofilms of C. albicans monospecies (left panel) or C. albicans-streptococci mixed-species (right panel). Biofilms were grown on the surface of three-dimensional models of oral mucosa under wet (media-submerged) conditions. X-Y isosurfaces (top panel) and 3-D reconstructions (bottom panels) of representative confocal laser scanning microscopy images are shown. C. albicans (green) was visualized after staining with a FITC-conjugated anti-Candida antibody. S. oralis (red) was visualized after fluorescence in situ hybridization (FISH) with a Streptococcus-specific probe conjugated to Alexa 546. Scale bar = 50 µm.

B: Sixteen-hour mucosal biofilms of C. albicans monospecies (left panel) or C. albicans-streptococci mixed-species (right panel). Biofilms were grown on the surface of three-dimensional models of oral mucosa under semidry (media limited to inoculum) conditions. X-Y isosurfaces (top panel) and 3-D reconstructions (bottom panels) of representative confocal laser scanning microscopy images are shown. C. albicans (green) was visualized after staining with a FITC-conjugated anti-Candida antibody. S. oralis (red) was visualized after fluorescence in situ hybridization (FISH) with a Streptococcus-specific probe conjugated to Alexa 546. Scale bar = 50 µm.