Table 2.

Growth percentages of Aspergillus nidulans strains in presence of NFAP and NFAP-8 Br-cAMP/caffeine combinations in in vitro broth microdilution test after 48 h of incubation at 37°C

NFAP/other compounds	0 µg/mL	50 µg/mL	100 µg/mL	200 µg/mL
FGSC A4
NFAP	100%	91% ± 6.0%	67% ± 6.7%	52% ± 3.2%
NFAP + 5 mmol/L 8-Br-cAMP	70% ± 1.6%	58% ± 4.2%***	57% ± 3.8%***	41% ± 3.2%***
NFAP + 20 mmol/L caffeine	71% ± 2.1%	74% ± 3.7%ns	61% ± 2.6%***	108% ± 1.5%***
∆pkaA
NFAP	100%	83% ± 11.5%	69% ± 5.3%	56% ± 6.1%
NFAP + 20 mmol/L caffeine	63% ± 6.5%	123% ± 7.9%***	195% ± 30.6%***	196% ± 3.0%***

The untreated control is taken as 100% of growth. The mean growth percentages and their standard deviations from three replicates (n = 3) are indicated in the cells. Significant differences (P-values) were determined based on the comparison with the growth percentages in the presence of 8-Br-cAMP or caffeine alone. ***: P < 0.0001; ^ns: no significant differences. 8-Br-cAMP: 8-bromoadenosine 3’,5’-cyclic monophosphate, NFAP: Neosartorya fischeri antifungal protein