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Western blot. Normal testis total protein was extracted from TEFNA biopsy using radioimmunoprecipitation assay (RIPA) buffer. 40ug of protein was separated and analyzed by Western blot on 10 % SDS-PAGE and probed with anti-Syce1 Ab (1:1000; NBP1-88971, Novus). A single band at the predicted size of Syce1, ~ 50 kDa, is evident, underscoring the specificity of this antibody to Syce1 on testicular tissue
