Fig. 2.

GJis delayed embryonic development (TLM observation). The mean time spent from the second cleavage to third cleavage, compaction, initiation of blastocoel formation, and initiation of hatching. Embryos were cultured with CZB (n = 41), 0.1 % DMSO (n = 37), oleamide 50 μM (n = 43), or heptanol 10 mM (n = 31). The vertical axis shows total cultured minutes from the second cleavage. p-value is for oleamide or 1-heptanol versus control. p < 0.001 (*), p < 0.05 (**) a: The time from the second cleavage to the third cleavage was significantly delayed with both GJis. b: Though the time to compaction was not significantly different with oleamide, embryos cultured with heptanol were significantly delayed. c: The time to initiation of blastocoel formation was significantly longer in the presence of both GJis. d: The difference in the time to start of hatching was not significant at all