Fig. 3.

Aberrant ICM divisions were observed in mouse embryos. a: Mouse blastocyst cultured with 50 μM oleamide. Plural strands (white arrowhead) from the ICM (*) were also observed. This aberrant ICM division was observed upon blastocoel formation. b: Mouse blastocyst cultured with 10 mM heptanol. Strands (white arrowhead) from the ICM (*) were observed. This aberrant ICM division was observed after a collapse. c: Human blastocyst frozen by vitrification on day 5, 3BB (blastocyst grading system introduced by Gardner and Schoolcraft in 1999), and thawed. Plural sticky strands (white arrowhead) from the ICM (*) were observed radially. This aberrant ICM division was observed after a collapse. Scale bar: 50 μm