Figure 4.

Submerged NHBE cells were treated with HKSA (5 × 10⁸ particles/ml) for up to 48 hours and cell lysates were collected at the indicated time points. (A) Cellular protein was collected for Western blot. Expressed bands for (B and C) phospho– and total NF-κB, (D and E) phospho– and total EGF receptor (EFGR), and (F and G) phospho– and total ERK1/2 were quantified using Image J software (NIH, Bethesda, MD) and normalized to internal control actin. Data in B–G represent mean ± SEM of three independent experiments, and images of Western blot are representative of three independent experiments. *P < 0.01 by t test when compared with media-only control.