Table 2.

Qualitative Comparison of Fluorescence-Activated Cell Sorting– and Magnetic-Activated Cell Sorting–Based Isolations from Lungs of Mice with Adenocarcinoma

	MACS	FACS
Total time of Isolation/mouse	5–6 h (possible to do simultaneous isolations from several mice)	3 h (allows for isolation from only one mouse at a time)
Yield	Comparatively higher yield	Lower yield
Viability	Higher viability*	Lower viability
Purity	Lower overall purity	Higher purity for EpCAMpos and CD31pos fractions

For definition of abbreviations see Table 1.

^*Epithelial cell viability higher when doing CD45/CD31 simultaneous depletion.