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. 2015 Jul 4;14:95. doi: 10.1186/s12934-015-0290-9

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© Christophe et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of surface expression of aDec on internalization of L. plantarum by DCs. a Monocyte derived hDCs were co-incubated with Lp-WT, Lp-Lip-aDec, Lp-CWA-aDec or Lp-LysM-aDec with or without addition of a free anti human DEC205 antibody. After incubation, the hDCs were washed and non-internalized bacteria were killed by treatment with gentamicin. The numbers of internalized bacteria were counted as CFU. Same amount of DCs was used in each experiment. b BMDCs were co-incubated with Lp-WT, Lp-Lip-aDec, Lp-CWA-aDec or Lp-LysM-aDec. After incubation, the BMDCs were washed and non-internalized bacteria were killed by treatment with gentamicin. The numbers of internalized bacteria were counted as CFU. Each point represents independent wells and results are presented as mean ± SEM. The results presented are from one experiment representative of three performed independently. Statistically significant differences are indicated as follows: *p < 0.05; **p < 0.01; ***p < 0.001.