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. 2015 Jul 4;14:95. doi: 10.1186/s12934-015-0290-9

Table 3.

Method for plasmid construction

Plasmid name Original plasmid References Restriction sites Primer used for aDEC amplification
pLip-aDec pLp_1452Inv [31] SalI, EcoRI LipaDecFW, LipaDecBW
pCWA-aDec pLp_0373sOFAcwa2 [36] SalI, MluI CwaaDecFW, CwaaDecBW
pLysM-aDec pLp_3014Inv [31] SalI, EcoRI LysMdecFW, LysMdecBW