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. 2015 Jul 4;15:135. doi: 10.1186/s12866-015-0471-z

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© Roszczenko et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Size exclusion profile of the purified HP0377 separated on a Superdex75 HiLoad 16/600 column (GE Healthcare) and monitored by absorbance at 280 nm. HP0377 elutes as a two peaks at 61 min, with an estimated mass of 48 kDa, consistent with the size of the homodimer and 66 min. with estimated mass of 24 kDa, consistent with the size of the monomer. The column was calibrated with Gel Filtration Standard (Bio-Rad): Thyroglobulin (670 kDa), γ-globulin (158 kDa), Ovalbumin (44 kDa), Myoglobin (17 kDa), Vitamin B12 (1.35 kDa). The relative positions of the chosen standards are marked with arrows