Table 1.
Bacterial strains and plasmids used in this study
| Name | Genotype or relevant characteristics | Origin |
|---|---|---|
| Helicobacter pylori strains | ||
| 26696 | H. pylori wild-type | ATCC |
| N6 | H. pylori wild-type | [24] |
| PR305 | N6 dsbI::aph | [31] |
| PR336 | dsbI + in trans complementant of dsbI::aph | [31] |
| PR378 | N6 hp0231::cat | [31] |
| PR397 | hp0231 + in trans complementant of hp0231::cat | [31] |
| MG100 | N6 hp0265::aph | This study |
| Escherichia coli strains | ||
| BL21 (DE3) | F- ompT hsdSB(rB-mB-) gal dcm lon | Novagen |
| Rosetta (DE3) LacIq | F- ompT hsdSB (rB- mB-) gal dcm pRARE (Cmr) | Novagen |
| TG1 | supE44 hsdΔ 5 thi Δ(lac- proAB) F’ [traD36 proAB + lacIq lacZΔM15] | [26] |
| Top10 | F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZ ΔM15 ΔlacX74 deoR nupG recA1 araD139 Δ(ara-leu)7697 galU galK rpsL(strr) endA1 λ- | Gibco BRL |
| JCB816 | MC1000 phoR λ102 | [72] |
| JCB817 | JCB816 dsbA::kan1 | [72] |
| JFC383 | JCB816 dsbC::kan | J.F. Collet Collection |
| FED126 | MC1000 ΔdsbD | [73] |
| JFC571 | JFC383/pHEL2 | This study |
| JFC572 | JCF383/pUWM500 | This study |
| JFC573 | JF383/pUWM399 hp0377 + | This study |
| PR501 | JCB817/pHEL2 | This study |
| PR502 | JCB817/pUWM399 hp0377 + | This study |
| PR539 | FED126/pUWM399 hp0377 + & pUWM536 | This study |
| PR540 | JCB816/pUWM399 hp0377 + & pUWM536 | This study |
| PR580 | FED126/pUWM399 hp0377 + | This study |
| PR581 | JCB816/pUWM399 hp0377 + | This study |
| General cloning/Plasmid vectors | ||
| pET28a | Kmr, IPTG inducible | Novagen |
| pGEM-T Easy | Apr; LacZa | Promega |
| pBlusescript SK II | Amr, LacZa | Stratagene |
| pHEL2 | Cmr E. coli/H. pylori shuttle vector | [74] |
| pMPM-K6Ω | Kmr,Spr; ori f1, ori P15A; arabinose inducible | [75] |
| pRY109 | Cmr | [76] |
| pUOA13 | KmR,TetR | [77] |
| pILL2150 | Cmr E.coli/H. pylori shuttle vector; IPTG inducible | [47] |
| Plasmids for mutagenesis | ||
| pUWM505 | pGEM-T Easy/hp0377::aph | This study |
| pUWM529 | pGEM-T Easy/hp0377::aph | This study |
| pUWM555 | pGEM-T Easy/hp0377::aph | This study |
| pUWM2019 | pBluescript II SK/hp0265::aph | This study |
| Plasmids for recombinant protein synthesis and purification | ||
| pUWM518 | pET28a/hp0377 | This study |
| pUWM2046 | pET28a/hp0377 C89S | This study |
| pUWM2065 | pET28a/hp0377 C92S | This study |
| pUWM2090 | pET24a/hp1227 | This study |
| pET28a-dsbA | pET28a/EcdsbA | J.F. Collet Collection |
| pET28a-dsbC | pET28a/EcdsbC | J.F. Collet Collection |
| pUWM525 | pET28a/hp0231 | [31] |
| Other plasmids | ||
| pUWM500 | pHEL2/hp0231 | [31] |
| pUWM336 | pHEL2/dsbI | [78] |
| pUWM399 | pHEL2/hp0377 | This study |
| pUWM389 | pGEM-T Easy/hp0231 | This study |
| pUWM536 | pMPM-K6Ω/hp0265 | This study |
| pUWM543 | pGEM-T easy/hp0377 | This study |
| pUWM544 | pGEM-T easy/hp0377 without promoter and signal sequence | This study |
| pUWM2045 | pGEM-T easy/hp0377 C89S | This study |
| pUWM2064 | pBluescript II SK/hp0377 C92S | This study |
| pUWM2073 | pBluescript II SK/hp0377 C25A | This study |