Table 4.
Relative miRNA expression levels of the 30 selected miRNAs between ER+ or ER− breast tumors and normal breast tissues
| Official Name | Normal breast tissue (n = 8) | ER+ breast tumors (n= 85) | ER+/Normal | ER− breast tumors (n= 68) | ER−/Normal | ||
|---|---|---|---|---|---|---|---|
| FC | p-value | FC | p-value | ||||
| 12 miRNAs up-regulated in ER+ compared to ER− | |||||||
| miR-190b | 1.0 (0.32-1.57) | 6.34 (0.71-32.3) | 6.34 | <0.0001 | 0.27 (0.02-6.23) | −3.70 | 0.0284 |
| miR-26a1 | 1.0 (0.77-4.06) | 0.34 (0.11-1.78) | −2.94 | <0.0001 | 0.09 (0.02-0.47) | −11.11 | <0.0001 |
| miR-342-5p | 1.0 (0.78-2.16) | 2.03 (0.29-19.4) | 2.03 | 0.0026 | 0.93 (0.10-3.65) | −1.07 | ns |
| miR-101-1 | 1.0 (0.69-8.05) | 0.30 (0.05-2.10) | −3.33 | <0.0001 | 0.15 (0.01-0.92) | −6.67 | <0.0001 |
| let-7b | 1.0 (0.71-1.69) | 0.53 (0.08-2.04) | −1.89 | 0.0007 | 0.31 (0.04-0.82) | −3.23 | <0.0001 |
| miR-30e | 1.0 (0.70-2.98) | 0.79 (0.12-4.57) | −1.27 | ns | 0.47 (0.10-2.28) | −2.13 | 0.0005 |
| miR-30c2 | 1.0 (0.69-1.72) | 0.54 (0.12-3.06) | −1.85 | 0.0032 | 0.34 (0.09-1.17) | −2.94 | <0.0001 |
| miR-143 | 1.0 (0.45-2.72) | 0.21 (0.03-1.40) | −4.76 | <0.0001 | 0.14 (0.02-0.74) | −7.14 | <0.0001 |
| miR-26b | 1.0 (0.68-4.38) | 0.49 (0.15-2.93) | −2.04 | 0.0034 | 0.34 (0.06-1.64) | −2.94 | 0.0001 |
| miR-376c | 1.0 (0.52-3.04) | 0.14 (0.02-1.55) | −7.14 | <0.0001 | 0.10 (0.02-0.83) | −10.00 | <0.0001 |
| miR-193b | 1.0 (0.43-1.79) | 1.44 (0.10-9,17) | 1.44 | ns | 1.00 (0.11-4.45) | 1.00 | ns |
| let-7a | 1.0 (0.73-1.79) | 0.58 (0.15-2.00) | −1.72 | 0.0017 | 0.47 (0.11-2.99) | −2.13 | 0.0002 |
| 8 miRNAs down-regulated in ER+ compared to ER− | |||||||
| miR-18a | 1.0 (0.49-3.33) | 0.50 (0.06-2.53) | −2.00 | 0.0074 | 1.12 (0.12-23.9) | 1.12 | ns |
| miR-654-3p | 1.0 (0.72-1.34) | 0.30 (0.05-2.68) | −3.33 | 0.0001 | 0.66 (0.04-17.8) | −1.51 | ns |
| miR-18b | 1.0 (0.47-4.45) | 0.50 (0.07-3.52) | −2.00 | 0.0063 | 1.04 (0.13-25.3) | 1.04 | ns |
| miR-155 | 1.0 (0.55-4.24) | 2.06 (0.52-10.9) | 2.06 | 0.0181 | 3.97 (0.35-32.0) | 3.97 | 0.0019 |
| miR-1244 | 1.0 (0.73-1.69) | 1.23 (0.16-5.11) | 1.23 | ns | 2.34 (0.35-25.3) | 2.34 | 0.0073 |
| miR-203 | 1.0 (0.51-2.43) | 0.88 (0.02-5.78) | −1.14 | ns | 1.51 (0.05-19.6) | 1.51 | ns |
| miR-92a1 | 1.0 (0.68-1.40) | 0.32 (0.10-1.17) | −3.12 | <0.0001 | 0.49 (0.09-8.95) | −2.04 | 0.0007 |
| miR-146a | 1.0 (0.54-3.71) | 0.70 (0.07-3.63) | −1.43 | ns | 0.95 (0.10-4.25) | −1.05 | ns |
| 10 miRNAs not differentially expressed in ER+ compared to ER− | |||||||
| miR-451 | 1.0 (0.32-11.1) | 0.17 (0.02-14.0) | −5.88 | 0.0009 | 0.14 (0.01-9.77) | −7.14 | 0.0003 |
| miR-21 | 1.0 (0.49-5.26) | 2.05 (0.41-16.6) | 2.05 | 0.0269 | 1.84 (0.21-9.53) | 1.84 | ns |
| miR-22 | 1.0 (0.38-4.51) | 0.71 (0.12-12.9) | −1.41 | ns | 0.66 (0.09-3.36) | −1.51 | ns |
| miR-494 | 1.0 (0.85-2.17) | 0.65 (0.02-6.96) | −1.54 | 0.0462 | 0.69 (0.02-9.03) | −1.45 | 0.0449 |
| miR-338-5p | 1.0 (0.79-1.78) | 0.84 (0.19-4.66) | −1.19 | ns | 0.96 (0.12-5.08) | −1.04 | ns |
| miR-19a | 1.0 (0.70-2.13) | 0.34 (0.03-2.61) | −2.94 | 0.0003 | 0.42 (0.02-14.4) | −2.38 | 0.0037 |
| miR-222 | 1.0 (0.63-2.30) | 0.39 (0.06-2.68) | −2.56 | 0.0001 | 0.50 (0.04-3.88) | −2.00 | 0.0015 |
| miR-206 | 1.0 (0.01-2.28) | 0.25 (0.02-7.71) | −4.00 | 0.0156 | 0.32 (0.01-2.74) | −3.12 | 0.0114 |
| miR-221 | 1.0 (0.65-1.92) | 0.40 (0.07-4.24) | −2.50 | 0.0004 | 0.53 (0.05-5.55) | −1.89 | 0.0098 |
| miR-891a | 1.0 (0.77-1.64) | 1.37 (0.14-8.43) | 1.37 | ns | 2.07 (0.37-15.5) | 2.07 | 0.0381 |
Results in ER+ and ER− breast tumors are expressed as the median (range) of miRNA level relative to normal breast tissues. For each miRNA, we report the fold-change (FC) between ER+ or ER− breast tumors and normal breast tissue and the p-value associated to Mann-Whitney’s test (ns for not significant)