Table 1.

Primer sequences for PCR, real-time PCR, methylation-specific PCR (MSP), quantitative MSP (Qmsp) and bisulfite (Bis.) sequencing analysis

Name	Sequence (5′-3)	Experiment
M-SOST-forward	GAATAGGTCGGGTTTAGTTTC	MSP, qMSP
M-SOST-reverse	ACCTCCCACGTACTAACGA	MSP, qMSP
U-SOST-forward	GGAATAGGTTGGGTTTAGTTTT	MSP, qMSP
U-SOST-reverse	CACCTCCCACATACTAACAA	MSP, qMSP
SOST-forward	CCGGAGCTGGAGAACAACAAG	RT-PCR
SOST-reverse	GGTGTGCTCCGGCCAGTGC	RT-PCR
Promoter SOST-forward	GGGACCAATGGGATTTCTTT	PCR
Promoter SOST-reverse	TGAGCTCCGGCTTTTAATTG	PCR
BSP SOST-forward	TTATTTGTTGGTGGGGTGATAA	Bis. sequencing
BSP SOST-reverse	ACAAAACCCAAACCTACTCTCC	Bis. sequencing