Fig. 1.

Effect of cycling exercise on plasma small EVs. Plasma samples were collected pre, post and 90 min post (90+) ergometer exercise, and 100,000×g pellets were prepared. Particle size and concentration were measured by NTA [(a) subjects a and b, age above 30] and protein content was analyzed by Western blotting [(b) subjects 1–6]. Western blot signals of Flot1 [(c) pre/post: n=6, 90+: n=5], Hsp/Hsc70 [(d) pre/post: n=6, 90+: n=5], Tsg101 [(e) pre/post: n=3, 90+: n=2; not detectable in all experiments] and IntαIIb [(f) pre/post: n=6, 90+: n=5] were quantified (**p<0.01, Wilcoxon–Kruskal–Wallis test). Subjects older than 30 years are marked in red (subject 2, 38 years; subject 5, 58 years).