Figure 3.

Plot of free R factor against resolution after refinement (all atom coordinates, individual B factors) to 2.8 Å resolution against the original data set (dashed black lines) and hybrid data sets (red lines) with scrambled intensities. The arrows indicate the beginning of the scrambled data at (a) 5.82 Å, (b) 4.62 Å, (c) 4.04 Å, (d) 3.67 Å, (e) 3.41 Å, (f) 3.21 Å, (g) 3.04 Å and (h) 2.91 Å. These cutoffs were chosen in accordance with the resolution shells used by phenix.refine to calculate the R _free values of the model.