Table 3.

Gene expression analysis in white adipose tissue (eWAT) of animals in Experiment 1

Gene name	Gene symbol	LFD	HFD	HFD/EGCG	HFD/PUFA	HFD/HP	ANOVA
Lipid metabolism
ATP citrate lyase	Acly	1.00 ± 0.23a	0.55 ± 0.03a	0.42 ± 0.07b	0.69 ± 0.0.11a	0.23 ± 0.23b	0.002
ELOVL family member 6	Elovl6	1.00 ± 0.18a	0.48 ± 0.10b	0.33 ± 0.06b	0.45 ± 0.08b	0.17 ± 0.03b	<0.0001
Fatty acid synthase	Fasn	1.00 ± 0.18	0.91 ± 0.11	0.71 ± 0.09	0.79 ± 0.11	0.50 ± 0.12	ns
Acetyl-coenzyme A carboxylase alpha	Acaca	1.00 ± 0.13a	0.44 ± 0.02b	0.52 ± 0.07b	0.62 ± 0.08b	0.40 ± 0.05b	<0.0001
Malic enzyme 1	Me1	1.00 ± 0.21a	0.71 ± 0.10ab	0.49 ± 0.06ab	0.79 ± 0.13ab	0.38 ± 0.06b	0.01
Patatin-like phospholipase domain-containing protein 2 (adipose triglyceride lipase)	Pnpla2	1.00 ± 0.07ab	1.14 ± 0.13a	0.60 ± 0.05b	1.04 ± 0.10a	0.88 ± 0.12ab	0.004
Carbohydrate metabolism
Glycogen synthase 2	Gys2	1.00 ± −0.17ab	1.19 ± 0.26ab	0.70 ± 0.10ab	1.67 ± 0.37a	0.66 ± 0.17b	0.02
Solute carrier family 2 (facilitated glucose transporter), member 4	Slc2a4	1.00 ± 0.07a	1.64 ± 0.13ab	1.54 ± 0.11ab	0.92 ± 0.08a	2.16 ± 0.37b	0.0002
Transketolase	Tkt	1.00 ± 0.12a	0.58 ± 0.06b	0.55 ± 0.06b	0.73 ± 0.10ab	0.48 ± 0.06b	0.0008
Adipokines
Serine (or cysteine) peptidase inhibitor, clade E, member 1	Serpine1	1.00 ± 0.22	1.36 ± 0.25	0.82 ± 0.14	0.79 ± 0.14	0.69 ± 0.14	ns
Haptoglobin	Hp	1.00 ± 0.13a	1.22 ± 0.16ab	1.23 ± 0.08ab	1.03 ± 0.08a	1.66 ± 0.21b	0.02
Retinol-binding protein 4, plasma	Rbp4	1.00 ± 0.14ab	1.45 ± 0.26a	0.49 ± 0.05b	1.42 ± 0.17a	0.55 ± 0.05b	<0.0001
Oxidative phosphorylation
NADH dehydrogenase (ubiquinone) Fe-S protein 1	Ndufs1	1.00 ± 0.06	0.97 ± 0.05	0.92 ± 0.05	1.04 ± 0.08	0.95 ± 0.05	ns
ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1	Atp5c1	1.00 ± 0.05	1.17 ± 0.06	0.95 ± 0.04	0.99 ± 0.08	0.99 ± 0.06	ns
Miscellaneous
Epithelial membrane protein 1	Emp1	1.00 ± 0.06	1.09 ± 0.14	1.37 ± 0.18	0.92 ± 0.14	1.17 ± 0.16	ns
Transformation-related protein 53-inducible nuclear protein 2	Trp53inp2	1.00 ± 0.07	1.57 ± 0.13	1.41 ± 0.15	1.27 ± 0.15	1.25 ± 0.14	ns
Thyroid hormone responsive SPOT14 homolog (rattus)	Thrsp	1.00 ± 0.12	1.00 ± 0.10	0.86 ± 0.13	1.36 ± 0.20	1.32 ± 0.17	ns
Protein phosphatase 1, regulatory (inhibitor) subunit 1A	Ppp1r1a	1.00 ± 0.07	0.98 ± 0.11	0.93 ± 0.07	1.32 ± 0.16	0.94 ± 0.11	ns
Cell death-inducing DNA fragmentation factor, alpha subunit-like effector A	Cidea	1.00 ± 0.13a	0.52 ± 0.09b	0.59 ± 0.06b	0.64 ± 0.09ab	0.45 ± 0.05b	0.001
Insulin-induced gene 1	Insig1	1.00 ± 0.15a	1.02 ± 0.10a	0.67 ± 0.10ab	1.00 ± 0.15a	0.42 ± 0.08b	0.003

Data are mean ± SEM (n = 7–8). Significant differences between groups were tested by one-way ANOVA and least-significant difference (LSD) post hoc comparisons (P < 0.05). Values not sharing a common letter are statistically different