Table 2.
Number of non-recombined and recombined non-rDNA- and rDNA-bearing chromosomes and their genome assignment in plants of F2-F4 generations derived from the allotetraploid F. pratensis × L. perenne hybrid
| Generation/plant no. | Chromosome ratio M+/M− | No. of non-recombined chromosomes | No. of recombined chromosomes | No. of recombined arms | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| M+ | M− | M+ | M− | m+ | m− | ||||||||
| Lp | Fp | Lp | Fp | Lp | Fp | Lp | Fp | Lp | Fp | Lp | Fp | ||
| F2-7 | 11:17 | 6 | 5 | 7 | 8 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
| F2-9 | 12:16 | 6 | 5 | 6 | 7 | 1 | 0 | 1 | 2 | 1 | 0 | 0 | 0 |
| F2-13 | 11:17 | 7 | 4 | 5 | 10 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
| F2-15 | 11:17 | 6 | 5 | 7 | 8 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
| F2-80 | 14:14 | 9 | 5 | 5 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| F2-126 | 13:15 | 9 | 4 | 5 | 9 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| F2-11 | 13:15 | 6 | 5 | 7 | 7 | 2 | 0 | 0 | 1 | 0 | 0 | 2 | 0 |
| F2-28 | 13:15 | 6 | 5 | 7 | 6 | 2 | 0 | 0 | 2 | 2 | 0 | 0 | 0 |
| F2-122 | 11:17 | 6 | 4 | 7 | 7 | 1 | 0 | 1 | 3 | 1 | 0 | 0 | 0 |
| F2-79 | 13:15 | 7 | 4 | 8 | 6 | 2 | 0 | 0 | 1 | 1 | 0 | 1 | 0 |
| F3-28 | 14:14 | 7 | 6 | 6 | 5 | 1 | 0 | 0 | 3 | 1 | 0 | 0 | 0 |
| F3-34 | 12:16 | 7 | 3 | 5 | 7 | 1 | 1 | 1 | 3 | 1 | 0 | 0 | 1 |
| F3-150 | 11:17 | 6 | 4 | 6 | 9 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 |
| F3-18 | 12:16 | 6 | 6 | 6 | 8 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
| F3-57 | 13:15 | 6 | 5 | 5 | 8 | 2 | 0 | 0 | 2 | 2 | 0 | 0 | 0 |
| F3-123 | 11:17 | 6 | 1 | 7 | 8 | 1 | 3 | 0 | 2 | 1 | 1 | 0 | 2 |
| F3-1 | 11:17 | 4 | 4 | 8 | 7 | 2 | 1 | 1 | 1 | 2 | 0 | 0 | 1 |
| F3-96 | 11:17 | 6 | 3 | 8 | 6 | 0 | 1 | 1 | 3 | 0 | 0 | 0 | 1 |
| F3-106 | 12:16 | 7 | 3 | 6 | 6 | 0 | 2 | 3 | 1 | 0 | 1 | 0 | 1 |
| F3-139 | 11:17 | 6 | 1 | 8 | 8 | 1 | 3 | 1 | 1 | 1 | 1 | 0 | 2 |
| F4-28 | 13:15 | 8 | 4 | 4 | 5 | 0 | 1 | 2 | 4 | 0 | 0 | 0 | 1 |
| F4-33 | 12:16 | 6 | 2 | 6 | 7 | 2 | 2 | 0 | 3 | 2 | 2 | 0 | 0 |
| F4-53 | 12:16 | 7 | 3 | 5 | 7 | 1 | 1 | 1 | 3 | 1 | 0 | 0 | 1 |
| F4-83 | 12:16 | 6 | 4 | 6 | 4 | 2 | 0 | 0 | 6 | 1 | 0 | 1 | 0 |
| F4-6 | 9:19 | 2 | 1 | 9 | 9 | 1 | 5 | 0 | 1 | 1 | 1 | 0 | 4 |
| F4-10 | 10:18 | 6 | 4 | 8 | 6 | 0 | 1 | 1 | 2 | 0 | 1 | 0 | 0 |
| F4-104 | 12:16 | 6 | 3 | 7 | 6 | 0 | 3 | 2 | 1 | 0 | 0 | 0 | 3 |
| F4-109 | 12:16 | 7 | 2 | 7 | 5 | 1 | 2 | 1 | 3 | 1 | 1 | 0 | 1 |
| F4-135 | 13:15 | 7 | 2 | 7 | 5 | 2 | 2 | 1 | 2 | 1 | 0 | 1 | 2 |
| F4-25 | 12:16 | 9 | 2 | 5 | 2 | 0 | 1 | 4 | 5 | 0 | 0 | 0 | 1 |
| Test resulta | n.s. | n.s. | P = 0.001 | n.s. | n.s. | n.s. | P = 0.001 | n.s. | n.s. | n.s. | n.s. | n.s. | P = 0.013 |
| Test resultb | n.a. | n.a. | n.a. | n.s. | P = 0.008 | P = 0.035 | n.s. | ||||||
Lp L. perenne chromosomes, Fp F. pratensis chromosomes, M+ Lp and Fp marked chromosomes, M− Lp and Fp non-marked chromosomes, m+ Lp and Fp chromosomal arms with rDNA locus, m− Lp and Fp chromosomal arms without rDNA locus, n.a. means not analyzed
n.s. P > 0.05
aThe distribution of M+/m+ and M−/m− values for non-recombined and recombined chromosomes as well as recombined arms between generations was compared, and significant differences between distributions for Lp and Fp were assessed using Pearson’s chi-squared test (P ≤ 0.05); statistically significant differences between generations were found both for Fp rDNA-carrying non-recombined and recombined chromosomes (at P = 0.001) and for Fp non-rDNA-carrying recombined arms (at P = 0.013)
bThe distribution of M+/m+ and M−/m− values for recombined chromosomes and arms between both genomes was compared, and significant differences between distributions for Lp and Fp were assessed using Pearson’s chi-squared test (P ≤ 0.05); statistically significant differences between Lp and Fp genomes were found with respect to the number of recombined chromosomes without marker (M−) (at P = 0.008), and also with respect to the number of recombined arms with marker (m+) (at P = 0.035)