Table 1.

The modified Gallyas method

Procedural step	Time (minutes)
(1) Potassium permanganate solution (0.3%)	10
(2) Oxalic acid solution (1%)	1
(3) Wash with water	5
(4) Distilled water
(5) Lanthanum nitrate solution (0.4%)	40
(6) Wash in distilled water (3 times)	1 (each)
(7) Alkali silver iodide solution	1
(8) Wash in 0.5% aqueous acetic acid (3 times)	1 (each)
(9) Physical developer solution	18–20
(10) Wash in 0.5% aqueous acetic acid (3 times)	1 (each)
(11) Gold chloride solution (0.5%)	5
(12) Lightly wash with water, distilled water
(13) Aqueous solution of sodium thiosulfate (1%)	1
(14) Wash with water, distilled water	1
(15) Counter stain (0.1% nuclear fast red)	3
(16) Dehydrate in alcohol, clear with xylene, mount in Entellan new®
Total time required	94