Table 1.

Experimental set-up of the day/night regimen for the study of bed bug (Cimex lectularius) behaviour, with methods and periods of stimulation in the test arena in mixed and split populations

	Mixed population		Split population
Stimuli	Human (Days 2–14)	Control (Days 2–14)	Human (Days 2–4)	Human (Days 5–9)	CO2/empty Petri disha (Days 10–14)
00.00–07.00 h	○	○	○	○	○
07.00–07.30 h	•	○	○	○	○
07.30–08.00 h	○	○	○	○	○
08.00–10.00 h	○	○	○	○	○
10.00–10.30 h	•	○	•	•	▪
10.30–11.00 h	○	○	○	○	○
11.00–13.00 h	○	○	○	○	○
13.00–13.30 h	•	○	○	•	⓪
13.30–14.00 h	○	○	○	○	○
14.00–16.00 h	○	○	○	○	○
16.00–16.30 h	•	○	○	•	▪
16.30–17.00 h	○	○	○	○	○
17.00–19.00 h	○	○	○	○	○
19.00–19.30 h	•	○	○	•	⓪
19.30–20.00 h	○	○	○	○	○
20.00–22.00 h	○	○	○	○	○
22.00–22.30 h	•	○	○	○	○
22.30–23.00 h	○	○	○	○	○
23.00–24.00 h	○	○	○	○	○
Repetitions	14 days	14 days	3 days	5 days	5 days
	n = 3	n = 3	n = 2	n = 2	n = 2

^aThe order of CO₂/empty Petri dish was alternated daily.

White represents day (white, red and infrared lights on) and grey shading represents night (only red and infrared lights on).

○, no stimuli; •, human presence; ⓪, empty Petri dish; ▪, Petri dish with dried ice to release CO₂.