Fig. 3.

Effects of hypoglycemia on the expression and distribution of claudin-5 and ZO-1 in bEnd.3 cells. Confluent bEnd.3 cell monolayers were exposed to 0.5 mM conditioned medium for 0 (control), 6, 12, and 24 h. Cultures were then stained with claudin-5 (red), ZO-1 (red) and DAPI (nuclear, blue) to examine protein expression, distribution, and cell morphology changes. N = 3