Table 1.
Effect of cytokines on ion transport in airway epithelial cells.
| Model system | Cytokine | Ion channel | Techniques | Principle findings | Reference | EPub |
|---|---|---|---|---|---|---|
| Rabbit | TGFβ | ENaC | 22Na+ efflux, electrophysiology, flow cytometry, surface biotinylation, and RT-PCR | TGFβ decreases ENaC-mediated sodium and fluid uptake | [25] | 2014 |
|
| ||||||
| Air liquid epithelial cell culture with human CFBE41o− and transfected cells | IL6, IL8, and CXCL1/2 | CFTR, TMEM16A | Electrophysiology, immunocytochemistry, human cytokine antibody array, and cell-surface ELISA | CFTR and TMEM16A decrease secretion of IL6, IL8, and CXCL1/2 | [24] | 2012 |
|
| ||||||
| Slc26a9-deficient mice | IL13 | Slc26a9 | Electrophysiology | IL13 increases Slc26a9-mediated Cl− secretion | [23] | 2012 |
|
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| BALB/c mice | IL13 | ENaC | Electrophysiology, RT-PCR | IL13 decreases ENaC expression | [22] | 2010 |
|
| ||||||
| Human bronchial epithelial cells | IL17A | Bicarbonate transport | Electrophysiology, measurement of surface and intracellular pH | IL17A increases HCO3 − secretion | [21] | 2009 |
|
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| Human nasal epithelial cells | IL13 | CFTR | Immunocytochemistry, western blot | IL13 increases CFTR expression | [20] | 2007 |
|
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| Primary culture of rat alveolar epithelial type II cells, primary culture of human alveolar epithelial type II cells | Il1β | ENaC | 22Na+ efflux, electrophysiology, western blot, and RT-PCR | IL1β decreases ENaC expression | [18] | 2005 |
|
| ||||||
| Rat alveolar epithelial cells | TNFα | ENaC | Electrophysiology, northern blot, and western blot | TNFα decreases ENaC expression | [19] | 2004 |
|
| ||||||
| Primary culture of rat alveolar epithelial type II cells, primary culture of human alveolar epithelial type II cells | TGFβ | ENaC | 22Na+ efflux, electrophysiology, western blot, and RT-PCR | TGFβ decreases ENaC expression | [15] | 2003 |
|
| ||||||
| Primary culture of human bronchial epithelial cells | IL4, IL13 | CFTR, ENaC | Electrophysiology, western blot | IL4 and IL13 increase CFTR expression IL4 and IL13 decrease ENaC expression |
[16] | 2002 |
|
| ||||||
| Primary culture of human bronchial epithelial cells | IFNγ, TNFα | ENaC, CFTR | Electrophysiology, western blot, and transepithelial fluid transport | IFNγ decreases CFTR expression IFNγ decreases ENaC-mediated sodium transport TNFα increases CFTR expression |
[17] | 2000 |