Fig. 3.

Purity of recombinant versions of three Dermanyssus gallinae GST proteins (rDeg-GST −1, −2 and −3). rDeg-GST-1 (lane A), rDeg-GST-2 (lane B) and rDeg-GST-3 (lane C) were produced as fusion proteins with N-terminal His-tags and SUMO peptides then affinity purified through a HisTRAP column(GE healthcare) and the imidazole removed by dialysis against 10 mM Tris, 0.5 M NaCl, pH 7.4. Four μg of each rDeg-GSTs were denatured and electrophoresed on a 4–12 % Bis-Tris Novex gel (Invitrogen). Proteins were visualized with SimplyBlue™ SafeStain and molecular masses of the rDeg-GSTs estimated by comparison with SeeBlue® Plus2 pre-stained protein standards (Invitrogen)