Fig. 1.

Distinct mRNA expression patterns and prognostic associations of CRABP1 and CRABP2 in breast tumors. a Comparison of CRABP1 and CRABP2 mRNA levels (based on normalized gene microarray signal intensity) in normal mammary tissues (n = 10) and human breast cancer subtypes (n = 176). b, c CRABP1 (b) and CRABP2 (c) mRNA levels in tumor tissues sampled from the 176 breast cancer patient cohort. The MT and GT numbers refer to individual patients. RNA levels were measured by real-time quantitative RT-PCR with GAPDH serving as the internal control. Expression levels from triplicate reactions are shown relative to MT633. d, e CRABP1 (d) and CRABP2 (e) mRNA levels in a panel of 11 breast cancer cell lines. RNA levels were analysed by real-time quantitative RT-PCR and shown as fold change relative to ZR-75-1. f Kaplan-Meier overall patient survival curves generated based on low and high CRABP1 mRNA levels determined by ROC analysis. g Kaplan-Meier overall patient survival curves generated based on low and high CRABP2 mRNA levels. n, denotes sample size; *, p < 0.05; **, p < 0.01