Fig. 3.

Associations of subcellular CRABP1 and CRABP2 levels with breast cancer patient survival and Ki67 immunoreactivity. CRABP1, CRABP2 and Ki67 protein levels were determined by immunohistochemical analysis of a TMA containing triplicate cores for each tumor from a 120 primary breast tumor cohort. a-b Association of cytoplasmic and nuclear CRABP1 levels with patient survival. c-d Association of cytoplasmic and nuclear CRABP2 levels with patient survival. e Positive correlation of cytoplasmic and nuclear CRABP1 protein levels with Ki67 immunoreactivity. f Positive correlation of CRABP1 and Ki67 mRNA levels based on gene microarray analysis. g No significant correlation was observed between subcellular CRABP2 levels and Ki67 immunoreactivity. h No correlation was evident between CRABP2 and Ki67 mRNA levels. HR, hazard ratio; p, statistical significance level; r, correlation coefficient. Scores: 0, negative; 1, weak; 2, intermediate; 3, strong. As the size of samples expressed CRABP1 is small in our TMAs, tumor samples were classified into “positive” and “negative” groups for survival analysis