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. Author manuscript; available in PMC: 2015 Jul 5.
Published in final edited form as: Methods Mol Biol. 2011;709:299–312. doi: 10.1007/978-1-61737-982-6_20

Fig. 1.

Fig. 1

Multiple exon skipping in dystrophic dogs. (a) Schematic outline of the protocol. A splice site mutation in intron 6 leads to deletion of exon 7 at mRNA level in dystrophic dogs. To restore the reading frame, two additional exons (exon 6 and exon 8) need to be skipped (removed) by three oligo cocktail of antisense. Exon 9 is known as alternative splice site. (b) RT-PCR and cDNA sequencing after exon skipping in dystrophic dogs. Left panel; RT-PCR reveals exon 6–9 skipped in-frame products (101 bp) in dystrophic dogs after the treatment of cocktail oligos. Alternative splice site Exon 9 is also mostly removed from the resulting mRNA. Right panel; Exon-skipping patterns are further confirmed by cDNA sequencing.