Table 3.
mRNA fold change in rectosigmoid mucosa from IBS-D patients relative to healthy controls
| Parameter Data: Mean (95% CI) | IBS-C Group | P IBS-C vs. Control | Whole IBS-D Group | P IBS-D vs. Control | IBS-D + high BA Excretion (n = 20) | IBS-D + Normal BA Excretion (n = 27) | P, high vs. Normal BA Excretion |
|---|---|---|---|---|---|---|---|
| Bile acid absorption and effects | |||||||
| SLC10A2 (ASBT) | 1.86 (0.49, 3.22) | 0.0759 | 2.71 (1.37, 4.06) | 0.0298 | 3.27 (0.92, 5.63) | 2.36 (1.06, 3.66) | |
| NR1H4 (FXR) | 1.64 (0.86, 2.41) | 0.0420 | 3.02 (1.95, 4.08) | 0.0081 | 2.98 (1.50, 4.46) | 3.04 (1.90, 4.19) | |
| FGFR4 | 1.14 (0.86, 1.41) | 0.2030 | 0.82 (0.69, 0.95) | 0.0463 | 0.82 (0.67, 0.95) | 0.82 (0.67, 0.98) | |
| GPBAR1 | 1.45 (1.02, 1.88) | 0.0178 | 0.93 (0.74, 1.12) | 0.2298 | 0.90 (0.70, 1.11) | 0.95 (0.75, 1.16) | |
| Ion transport mechanisms | |||||||
| GUCA2B | 1.45 (0.56, 2.34) | 0.3359 | 3.81 (2.25, 5.36) | 0.00016 | 3.78 (1.99, 5.56) | 3.83 (2.23, 5.42) | |
| PDZD3 | 1.14 (0.00, 2.48) | 0.0099 | 2.51 (1.88, 3.14) | 0.000001 | 2.49 (1.72, 3.17) | 2.56 (1.88, 3.23) | |
| P2RY4 | 4.27 (1.45, 7.09) | 0.0016 | 11.34 (4.11, 18.58) | 0.000001 | 9.30 (2.05, 16.56) | 13.14 (5.01, 21.26) | |
| VIP | 3.21 (1.61, 4.80) | 0.0008 | 0.44 (0.24, 0.63) | 0.0030 | 0.46 (0.20, 0.73) | 0.42 (0.20, 0.63) | |
| SLC6A4 | 1.97 (0.73, 3.21) | 0.0726 | 2.75 (1.30, 4.21) | 0.0104 | 2.44 (0.71, 4.17) | 3.01 (1.38, 4.64) | |
| Immune functions | |||||||
| TNFSF15 | 1.17 (0.50, 1.84) | 0.1901 | 0.69 (0.48, 0.90) | 0.0185 | 0.55 (0.33, 0.77) | 0.81 (0.56, 1.06) | 0.10 |
| C4BPA | 1.97 (0.76, 3.17) | 0.0189 | 3.73 (2.37, 5.08) | 0.00003 | 4.14 (2.27, 6.00) | 3.45 (2.02, 4.88) | |
| CCL20 | 1.43 (0.35, 2.51) | 0.7339 | 0.23 (0.10, 0.36) | 0.000001 | 0.22 (0.08, 0.35) | 0.24 (0.10, 0.38) | |
| IFIT3 | 0.92 (0.33, 1.50) | 0.3555 | 0.84 (0.38, 1.30) | 0.0279 | 0.90 (0.39, 1.42) | 0.79 (0.35, 1.23) | |
| Barrier functions | |||||||
| TJP 1 (ZO-1) | 1.64 (1.14, 2.14) | 0.0070 | 0.76 (0.57, 0.96) | 0.0457 | 0.76 (0.57, 0.96) | 0.92 (0.72, 1.11) | 0.06 |
| OCLN | 1.77 (1.25, 2.30) | 0.0017 | 1.19 (0.87, 1.51) | 0.2900 | 1.12 (0.79, 1.45) | 1.25 (0.91, 1.59) | |
| CLDN1 | 1.20 (0.69, 1.70) | 0.7099 | 0.59 (0.39, 0.80) | 0.0004 | 0.63 (0.40, 0.86) | 0.57 (0.35, 0.78) | |
| RBP2 | 1.14 (0.04, 2.25) | 0.1005 | 1.78 (1.16, 2.40) | 0.0031 | 1.72 (1.08, 2.36) | 1.83 (1.13, 2.52) | |
| FN1 | 1.33 (0.77, 1.90) | 0.4134 | 0.35 (0.23, 0.47) | 0.00001 | 0.28 (0.16, 0.40) | 0.40 (0.26, 0.54) | 0.10 |
| TFF1 | 3.34 (0.00, 7.22) | 0.1918 | 1.17 (0.72, 1.63) | 0.8739 | 1.12 (0.63, 1.61) | 1.21 (0.72, 1.70) | |
Bolded data show univariately significant fold changes (without correction for false detection resulting from multiple comparisons) relative to healthy controls. The decreased expression is indicated by italics. Bolded P values reflect the comparison of whole IBS-D group vs. control corrected for FDR (Bonferroni correction) so that P < 0.00263 is statistically significant. There were no significant differences (with only few P values ≤0.10, without FDR correction) in mRNA fold changes in IBS-D patients with high vs. low fecal BA excretion; all other P values were >0.20.