Fig. 4.

Nrf2 regulates club cell function. A: oxidative damage to macromolecules was measured via quantification of protein carbonyls and lipid peroxidation in lung homogenates of CC10-Keap1^−/− and Keap1^fl/fl mice. B: airway leakiness was assessed via quantification of albumin in the cell-free BAL fluid by the Albumin Blue Fluorescent Assay Kit (Active Motif). Values are means ± SE; N = 7–8 mice per group. C: Zonula occludens-1 (Zo-1) and E-cadherin intensity were quantified in the airway epithelium of OVA-sensitized/challenged Keap1^fl/fl and CC10-Keap1^−/− mice, and normalized to background levels using Image J. Quantification was performed on a minimum of 4 airways per mouse. *P < 0.05 by ANOVA. †P < 0.05 by Student's t-test. D: representative ×200 images of airways from OVA-sensitized/challenged Keap1^fl/fl and CC10-Keap1^−/− mice immunostained for Zo-1. Arrows indicate epithelial cells that do not express Zo-1. SM, smooth muscle.