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CLI patients exhibit nitric oxide (NO) deficiency in the ischemic limb. A–E: nitrite, RSNO, NO-heme, cGMP, and nitrate in gastrocnemius muscle from CLI patients and controls. F: representative Western blot of gastrocnemius cGMP-dependent protein kinase 1 (PKG-1). G and H: PKG-1 RNA message and PKG-1 protein expression. Numbers inside circles denote number of patients.
