Fig. 3.

Cooperation between the FOXA and GATA transcription factor families. A: de novo motif analysis of the combined FOXA2 peaks in Caco2 cells within a 200 bp window. B: incidence of GATA motifs measured as a function of distance from the center of the FOXA2 ChIP-seq peaks. C: UCSC genome browser views of the GATA-4 and GATA-6 loci showing FOXA2 binding sites in Caco2 cells. D: quantification of GATA-4 protein levels, from Western blots, in cells treated with a NC siRNA or FOXA1/A2 targeting siRNAs (left). **P < 0.01. Data are normalized to β-tubulin, as an endogenous loading control. On the right are representative Western blots of GATA-4 and GATA-6 respectively in cells treated with Neg Cont or FOXA1/A2 siRNAs. β-Tubulin is shown below.