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Primers used for quantitative and semi-quantitative (semi-Q) PCR experiments
| Forward primer (5′–3′) | Reverse primer (5′–3′) | Annealing temperature (°C) | |
|---|---|---|---|
| Gr68a (qPCR) | CCAAGGTGATACCGAGGAGGAGA | TCGTGAAGAGTGCGAAAGTG | 60 |
| Gr68a (semi-Q PCR) | CCAAGGTGATACCGAGGAGA | CATTGGCCAGCAGATACTCA | 55 |
| CG6024 | CCAAGGTGATACCGAGGAGA | TCATGAAGAGTGCGAAAGTG | 60 |
| NPF | GCGAAAGAACGATGTCAACAC | TGTTGTCCATCTCGTGATTCC | 60 |
| rp49 | CCAAGGACTTCATCCGCCACC | GCGGGTGCGCTTGTTCGATCC | 55 |
| RMCE vector | GTACTGACGGACACACCGAAG | GGATCAACTACCGCCACCT | 52 |
