Table 2.
Detection of SCSMV from field sugarcane samples by real-time qRT-PCR with SCSMV-QPCR-P2 probe and conventional RT-PCR.
| Varieties | Sampling location | qRT-PCR | Conventional RT-PCR | ||||
|---|---|---|---|---|---|---|---|
| Ct value | Copies/μL | Result | SCSMV | SrMV | SCMV | ||
| FN38 | Fuzhou, Fujian | 30.53 ± 0.28 | 8.2 × 102 | + | — | +, [KC984957] | — |
| FN15 | Fuzhou, Fujian | na | na | − | — | +, [KC984956] | — |
| ROC22 | Fuzhou, Fujian | 30.37 ± 0.16 | 8.9 × 102 | + | — | +, [KC984964] | +, [KC899320] |
| FN39 | Zhangzhou, Fujian | na | na | − | — | +, [KC984958] | — |
| ROC22 | Guangzhou, Guangdong | na | na | − | — | +, [KC984980] | — |
| F160 | Suixi, Guangdong | 20.51 ± 0.33 | 5.1 × 105 | + | +, [KJ944725] | +, [KJ944744] | — |
| GT31 | Suixi, Guangdong | 33.51 ± 0.34 | 1.2 × 102 | + | +, [KJ944729] | +, [KJ944745] | — |
| LC05-136 | Suixi, Guangdong | na | na | − | — | +, [KJ944746] | — |
| ROC22 | Suixi, Guangdong | 32.68 ± 0.42 | 2.0 × 102 | + | +, [KJ944727] | +, [KJ944741] | — |
| YZ05-49 | Suixi, Guangdong | 19.34 ± 0.14 | 1.1 × 106 | + | +, [KJ944728] | +, [KJ944742] | — |
| YZ07-2384 | Zhanjiang, Guangdong | 16.72 ± 0.23 | 5.8 × 106 | + | +, [KJ944726] | +, [KJ944736] | — |
| GT11 | Wangmo, Guizhou | 32.55 ± 0.30 | 2.2 × 102 | + | +, [KC985070] | +, [KC985029] | — |
| GZ18 | Wangmo, Guizhou | 31.39 ± 0.5 | 4.6 × 102 | + | +, [KC985071] | — | — |
| YZ03-45 | Wangmo, Guizhou | 32.21 ± 0.15 | 2.7 × 102 | + | +, [KC985072] | +, [KC985030] | — |
| YG35 | Kaiyuan, Yunnan | 18.42 ± 0.36 | 1.9 × 106 | + | +, [KC985085] | +, [KC985056] | — |
| YN01-58 | Kaiyuan, Yunnan | 31.26 ± 0.25 | 5.0 × 102 | + | +, [KC985086] | +, [KC985061] | — |
| YZ05-211 | Kaiyuan, Yunnan | 21.19 ± 0.29 | 3.3 × 105 | + | +, [KC985090] | +, [KC985064] | — |
| YT93-159 | Lincang, Yunnan | 32.59 ± 0.31 | 2.1 × 102 | + | — | +, [KC985060] | — |
| FN15 | Danzhou, Hainan | 32.35 ± 0.22 | 2.5 × 102 | + | — | +, [KC985031] | — |
| YT55 | Danzhou, Hainan | 31.52 ± 0.27 | 4.2 × 102 | + | — | +, [KC985038] | — |
| ROC22 | Haikou, Hainan | 32.07 ± 0.07 | 3.0 × 102 | + | +, [KC985074] | — | — |
| YZ05-49 | Lingao, Hainan | 16.44 ± 0.27 | 6.9 × 106 | + | +, [KC985075] | +, [KC985041] | — |
| GT02-281 | Beihai, Guangxi | 32.13 ± 0.19 | 2.9 × 102 | + | +, [KC985069] | +, [KC984999] | — |
| FN1110 | Bingyang, Guangxi | na | na | − | — | +, [KJ944748] | — |
| YT93-159 | Bingyang, Guangxi | na | na | − | — | +, [KJ944750] | +, [KJ944720] |
| FN39 | Laibin, Guangxi | 32.27 ± 0.32 | 2.6 × 102 | + | +, [KJ944735] | +, [KJ944782] | — |
| ROC16 | Laibin, Guangxi | 32.17 ± 0.21 | 2.8 × 102 | + | — | +, [KJ944757] | — |
| ROC22 | Laibin, Guangxi | 29.60 ± 0.12 | 1.5 × 103 | + | +, [KJ944731] | +, [KJ944764] | — |
| YG46 | Laibin, Guangxi | 32.92 ± 0.35 | 1.7 × 102 | + | +, [KJ944734] | +, [KJ944774] | — |
| YR06-189 | Laibin, Guangxi | 32.89 ± 0.46 | 1.8 × 102 | + | — | +, [KJ944758] | — |
| GT05-3084 | Longan, Guangxi, | na | na | − | — | +, [KJ944820] | — |
| GT07-994 | Liuzhou, Guangxi | na | na | − | — | +, [KJ944814] | — |
| ROC22 | Liuzhou, Guangxi | na | na | − | — | +, [KJ944815] | — |
| FN15 | Shanglin, Guangxi | na | na | − | — | +, [KJ944752] | — |
| ROC22 | Shanglin, Guangxi | na | na | − | — | +, [KJ944754] | — |
Positive result (+) if Ct ≤ 35, negative result (−) if Ct > 35.
GenBank acc. no. of these sequences from conventional RT-PCR product were in brackets.
na: data not available.