Table 1.

Antibodies employed for each ELISA.

ELISA	Detection-antibody	Dilution
Anti-β2GP1-IgG	HRP-conjugated rabbit F(ab′) 2 anti-human-IgG∗	1 : 20.000
Anti-β2GP1-IgG subclasses	HRP-conjugated mouse anti-human IgG1, 2, 3 and 4 (Clones HP6001, HP6002, HP6050 & HP6025)∗	1 : 20.000
Anti-β2GP1-IgM	HRP-conjugated goat anti-human-IgM∗	1 : 20.000
Anti-β2GP1-bound C3c	HRP-conjugated sheep anti-C3c∗∗	1 : 500
Anti-β2GP1-bound C1q	HRP-conjugated sheep anti-C1q∗∗	1 : 500

∗: purchased from Southern Biotech, Birmingham, Alabama, USA.

∗∗: purchased from Abcam, Cambridge, United Kingdom.

HRP = horseradish peroxidase.