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. 2015 Jun 22;2015:747645. doi: 10.1155/2015/747645

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Copyright © 2015 Pier Paolo Piccaluga et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Double-staining immunofluorescence analysis of IFI16/BCL-6 and IFI16/BLIMP1 protein expression levels. Double-staining immunofluorescence analysis demonstrated the inverse relationship between IFI16 (green) and either BCL6 (red; (a-b) magnification 200x and 100x, resp.), or BLIMP1 (red; (c-d), magnification 100x and 400x, resp.). The latter, in particular, showed a mutually exclusive expression patterns with IFI16. Specifically, plasma cells in panel (d) were BLIMP1+/IFI16−, while the surrounding lymphocytes were BLIMP1−/IFI16+. In (e) (magnification 400x) double-staining immunofluorescence analysis showed the coexpression of BLIMP1 (red) and plasma cell marker CD138 (green). These micrographs were obtained using an Olympus BX61 microscope equipped with an Olympus DP-70 digital camera; image acquisition, evaluation, and color balance were performed using Cell^∧F software.