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. 2015 Jun 15;4(6):e159. doi: 10.1038/oncsis.2015.10

Figure 2.

Figure 2

Suppression of DCTPP1 markedly inhibits the proliferation of MCF-7 in vitro. (a) The suppression of DCTPP1 expression in DCTPP1-knockdown MCF-7 cells was determined by western blotting (upper panel) and real-time quantitative PCR with β-actin as internal reference (lower panel). (b) Proliferative assay was performed to examine the effect of DCTPP1-knockdown on cell proliferation in vitro by using CCK-8 detection kit. (c) Soft agar colony assays were performed and representative images from the assays were shown (upper panel). Statistical plots were conducted from >3 randomly selected fields (lower panel). (d) Cell cycles of MCF-7 cells were determined by flow cytometry and statistical results were indicated to compare the effects of DCTPP1 expression on cell cycle. (e) The overexpression of DCTPP1 in transfected MDA-MB-231 cells was confirmed by immunoblotting (left panel) and real-time PCR (right panel). (f) CCK-8 assay was performed to detect the proliferation rate of DCTPP1 overexpressing and control cells. All the values shown were represented as the means±s.d. (***P⩽0.001, **P⩽0.01).