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. 2015 Jun 15;4(6):e159. doi: 10.1038/oncsis.2015.10

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Copyright © 2015 Macmillan Publishers Limited

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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DCTPP1 modulates intracellular 5-methyl-dCTP concentration. The concentration of intracellular dCTP and 5-methyl-dCTP was determined by LC-MS assay based on the concentration of internal standards. Representative MRM chromatograms of the dCTP calibrators (a, left panel) and 5-methyl-dCTP calibrators (b, left panel) was shown.The calibration curves of dCTP and 5-methyl-dCTP were shown in right panel of (a and b), respectively. The concentration of dCTP and 5-methyl-dCTP in DCTPP1-deficient MCF-7 cells (c) and overexpressed MDA-MB-231 cells (d) was calculated accordingly. The concentration of dCTP and 5-methyl-dCTP in mammospheres formed by DCTPP1 knockdown MCF-7 (e) and DCTPP1-overexpressed MDA-MB-231 cells (f) were also measured by LC-MS assays. All data were collected from at least three independent experiments and each experiment was performed in triplicate.