Table 2. Kinetic parameters of DCTPP1 protein.
| Substrate | Vmax | kcat | Km | Kcat/Km |
|---|---|---|---|---|
| (d)NTP | nmol/min/μg | min-1 | μM | min-1mM-1 |
| ATP | NR | NR | NR | NR |
| UTP | NR | NR | NR | NR |
| GTP | NR | NR | NR | NR |
| CTP | NR | NR | NR | NR |
| dATP | 0.39±0.01 | 8.49±0.27 | 292.8±18.07 | 28.99 |
| dTTP | 0.30±0.01 | 6.49±0.22 | 196.10±16.27 | 33.09 |
| dGTP | NR | NR | NR | NR |
| dUTP | 0.22±0.01 | 4.86±0.15 | 240.6±19.07 | 20.20 |
| dCTP | 1.63±0.05 | 22.02±0.43 | 152.3±10.91 | 144.58 |
| 5-Methyl-dCTP | 0.99±0.03 | 21.70±0.60 | 85.03±7.02 | 255.20 |
| 5-Br-dCTP | 1.38±0.03 | 30.28±0.66 | 108.9±6.48 | 278.05 |
| 5-I-dCTP | 1.18±0.02 | 25.83±0.05 | 94.40±5.20 | 273.62 |
Abbreviation: NR, no reaction.
Standard assay was conducted as described in 'Materials and methods'. The results were analyzed from the reactions that contained nine different concentrations of each substrate. All data were presented as mean±s.d. from at least three independent experiments and each experiment was performed in triplicate. The bold values represent DCTPP1 expression is associated with corresponding clinical parameters.