Fig. 3.
Depleting intracellular glutathione had little effect on cell growth and clonogenic survival when coupled with TAK1 inhibition. Cells were pretreated with 100 µM BSO for 8 h to deplete intracellular glutathione, then treated with 5 µM 5Z-7-oxozeaenol. (A) After 24 h of treatment, cells were scrape harvested and total intracellular glutathione levels, shown as normalized to vehicle control, were assayed. (B) Cells were plated at time 0 and treatment applied at 24 h; cell counts were obtained at 48, 72, and 96 h after plating to determine cell growth rate. (C) After 24 h of treatment, cells were detached using TrypLE Express and plated at low density. 14 days later, colonies were fixed, stained, and counted to determine the rate of clonogenic survival. Values represent mean±1 SEM of at least 3 separate experiments. #P-value<0.01 versus control. *P-value<0.01. **P-value<0.05.