Fig. 5.

Auranofin inhibits thioredoxin reductase (TrxR) activity and enhances toxicity of TAK1 inhibition in both HCT 116 and SW 620 cells. SW 620 cells were pretreated with 500 nM auranofin, then with 5 µM 5Z-7-oxozeaenol. (A) Cells were plated at time 0 and treatment applied at 24 h; cell counts were obtained at 48 and 72 h after plating to determine cell growth rate. (B) After 24 h of treatment, cells were detached using TrypLE Express and plated at low density. 14 days later, colonies were fixed, stained, and counted to determine the rate of clonogenic survival. Errors represent ±1 SEM of at least 3 separate experiments. #P-value<0.01 versus control. ##P-value<0.05 versus control. *P-value<0.01. **P-value<0.05.