. 2015 Jun 18;8(2):351–365. doi: 10.3390/ph8020351

Table 2.

Primer sets used in this study.

Target Organism		Sequence (5' to 3')	Annealing
Target Organism		Sequence (5' to 3')	Temperature (°C)
Bifidobacterium	Forward	CTCCTGGAAACGGGTGG	60
Bifidobacterium	Reverse	GGTGTTCTTCCCGATATCTACA	60
Lactobacillus	Forward	CTTGTACACACCGCCCGTCA	57
Lactobacillus	Reverse	CTCAAAACTAAACAAAGTTTC	57
Bacteroides	Forward	ATAGCCTTTCGAAAGRAAGAT	50
Bacteroides	Reverse	CCAGTATCAACTGCAATTTTA	50
C. coccoides	Forward	AAATGACGGTACCTGACTAA	50
C. coccoides	Reverse	CTTTGAGTTTCATTCTTGCGAA	50
C. leptum	Forward	GCACAAGCAGTGGAGT	50
C. leptum	Reverse	CTTCCTCCGTTTTGTCAA	50
Enterobacteriaceae	Forward	CATTGACGTTACCCGCAGAAGAAGC	58
Enterobacteriaceae	Reverse	CTCTACGAGACTCAAGCTTGC	58
Enterococcus	Forward	CCCTTATTGTTAGTTGCCATCATT	56
Enterococcus	Reverse	ACTCGTTGTACTTCCCATTGT	56
Total bacteria	Forward	ACTCCTACGGGAGGCAGCAGT	60
Total bacteria	Reverse	GTATTACCGCGGCTGCTGGCAC	60