Figure 8. Localization of the paternal X- and Y-linked rDNA locus to the telophase bridge in female and male embryos fathered by the HP1E mutant (PEL embryos).
Representative images of FISH analyses of first zygotic telophase in (A) wild-type and (B) PEL embryos using (C) probes that recognize the Y-specific satellite AATAC (to determine sex of embryos) and rDNA (the intergenic spacer ‘IGS’ sequence). (D) Quantification of FISH signal in the first telophase bridge in male and female PEL embryos. Data are reported as ‘obs/total/(%)’ where ‘obs’ = number of embryos observed with the probe appearing in the telophase bridge, ‘total’ = total number of embryos sampled per probe, and ‘(%)’ = obs × 100/total.