. 2015 Jun 8;112(26):7972–7977. doi: 10.1073/pnas.1500625112

Table S1.

Actin-activated ATPase activity of Dicty S1dC constructs

Labeling site	Unlabeled		BSL labeled
Labeling site	V_max (s⁻¹)	K_ATPase (µM)	V_max (s⁻¹)	K_ATPase (μM)	Spins/protein
492.496-BSL (relay helix)	2.32 ± 0.10	7.77 ± 0.75	4.05 ± 0.30	15.40 ± 1.60	0.97
325.329-BSL (helix HK)	5.33 ± 0.30	15.06 ± 1.19	3.85 ± 0.99	22.00 ± 7.06	0.87
639.643-BSL (helix HW)	3.75 ± 0.18	13.43 ± 1.51	2.05 ± 0.06	11.80 ± 0.78	0.96

V_max is the activity at saturating actin, and K_ATPase is the actin concentration needed for half-maximal activation (V = V_max/2). Errors are ±SEM (n = 3). Label-to-protein ratios were determined by spin counting.