Fig. 3.

BIS1 overexpression boosts MIA production in stably transformed C. roseus suspension cultures. (A) Expression analysis in transformed cells by RNA-blot hybridization analysis showing the effect of constitutive overexpression of BIS1 on selected biosynthetic genes in three independent cell suspension cultures of C. roseus. RPS9 was used as a reference gene. (B) Accumulation of (seco)-iridoid and MIA compounds in the BIS1-overexpressing cell lines. Metabolite levels are indicated in mg·g^–1 dry weight. The error bars designate SE of the mean (n = 3). In control cell lines, no detectable levels of these compounds accumulated.