Fig. 3.
Optogenetic stimulation of IL and induction of Fos+ labeling. Control (rAAV2-GFP) or active (rAAV2-ChR2-eYFP) viral vectors were infused into the IL-PFC, and 2 wk were allowed for viral infection. (A) Location of viral infusions and conditions for in vivo optogenetic stimulation of IL. Both rAAV2-GFP and rAAV2-ChR2-eYFP animals received laser stimulation. (B) Influence of laser stimulation on Fos+ cell labeling. Representative images of Fos+ expression after photostimulation of IL in rAAV2-ChR2-eYFP and rAAV2-GFP control animals (×20). (Scale bar, 50 μm.) Optical stimulation in IL significantly increased the number of Fos+ cells in IL (t16 = 9.760, P < 0.01).