Fig. S5.

Influence of optogenetic stimulation of IL-PFC on Fos+ cell labeling in the subregions of medial PFC. (A) Map of IL and PrL in the medial PFC of rat. (B and C) Expression of active virus (rAAV2-ChR2-eYFP) in deep layers of IL and location of optic fiber. (C) Stimulation of IL in rats infused with either control virus (rAAV-GFP) or active virus (rAAV2-ChR2-eYFP) (20 min, 10 Hz, 15 ms pulse width; 1 min on and 1 min off) increases the density of Fos+ cells in the IL as well as the PrL. (Inset) High-magnification (20×) view of Fos immunolabeling in IL. **P < 0.05 compared with control virus by two-tailed t test. ACC, anterior cingulate; CC, corpus callosum; IL, infralimbic; PrL, prelimbic.