Fig. S7.

Actin and poly-l-proline binding residues exhibit relatively high double difference values. Residues that have Avg-Abs-DD values of 0.3 Å or greater that are also engaged in actin binding (V119, H120, G122, and K126) or poly-Pro binding (W4, Y7, H134, and S138) are mapped onto the structure of PFN1 WT in magenta. All other residues with Avg-Abs-DD values of 0.3 Å or greater are highlighted in green. Residues with Avg-Abs-DD values between chain A and chain B of M114T 0.3 Å or greater (Fig. S6D) were excluded from this analysis. Residues not used in this analysis are colored black.