Table S1.

Crystallographic and refinement statistics of human PFN1 structures

Statistics	WT	E117G	M114T
Resolution, Å	2.160	2.170	2.230
Space group	C121	C121	P6
a, Å	74.26	73.65	81.69
b, Å	31.84	31.71	81.69
c, Å	61.02	60.54	65.35
Α, °	90	90	90
Β, °	122.66	122.03	90
Γ, °	90	90	120
Z	1	1	2
Rmerge, % linear	0.075	0.036	0.147
I/sigma	13.3	12.2	12.4
Completeness, %	99.28	99.49	99.58
Total no. of reflections	20,783	16,453	76,801
No. of unique reflections	6,416	6,422	12,156
Rfactor, %	0.2159	0.1965	0.1952
Rfree, %	0.2469	0.2139	0.2383
RMSD in bond lengths, Å	0.002	0.003	0.003
RMS angle, °	0.62	0.67	0.61
Temperature, °C	−80	−80	−80
Residues missing	1, 2, 57–62, 92–96	1, 2, 59–62, 81,82, 93–95, 140
Chain A			1, 93–97
Chain B			1, 13, 91–97
PDB ID code	4X1L	4X1M	4X25