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qPCR analyses of R. felis transmission by An. gambiae through cotton used for sucrose feeding, after meals consisting of infected blood or infected cellular media
| Trial | DPI* | Positive/no. cotton (%) | Ct in qPCR1 | Ct in qPCR2 |
| Infected with blood (trial 1) | D1–D3 | 0 | 0 | 0 |
| D4–D8 | 1/8 (12.5) | 34.7 | 35.9 | |
| D9–D15 | 1/5 (20) | 35.5 | 36.6 | |
| Infected with cellular media (trial 2) | D1–D14 | 1/2 (50) | 32.5 | 35.5 |
Cotton was maintained during that period, and then removed to be tested.
