Table 4.
Detection of R. felis by qPCR in the blood of healthy mice on which An. gambiae mosquitoes were allowed to feed 14 d (experiments 1–3) or 12 d (experiments 4 and 5) after having been allowed to feed on mice inoculated by R. felis
| Experiment (E) | Mouse (M) | Ct obtained by qPCR in blood mice (qPCR1/qPCR3)* | No. mosquito specimens that fed on mice/no. qPCR positive (%) | ||
| D1 | D2 | D3 | |||
| E1 | M1 | N | 32/33 | N | ND |
| M2 | N | N | N | ND | |
| E2 | M1 | N | N | N | 18/27 (66) |
| E3 | M1 | 30/32 | N | N | 14/43 (32)** |
| M2 | N | N | N | ||
| E4 | M1 | N | 32.5/31 | N | 12/49 (24) |
| E5 | M1 | 33/34 | N | N | 14/43 (32) |
| M2 | N | 35 /34 | N | 9/41 (21) | |
*
The final volume of extracted DNA was 50 µL, and qPCR was performed with 5 µL of this extract. N, negative; ND, not done.
**
In E3, M1 and M2 were exposed together simultaneously to 43 mosquitoes.