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. 2015 Jun 25;6:7526. doi: 10.1038/ncomms8526

Figure 5. Ii-dependent recruitment of myosin IIA to the front of DCs regulates macropinosome dynamics.

Figure 5

(a) Sequential spinning disc images (× 60, middle plan, one image every 10 s) of myosin IIA-GFP knock-in WT and Ii−/− immature DCs migrating in microchannels filled with 10 kDa AF647-Dextran. (b) Sequential spinning disc images (× 60, middle plan, one image every 10 s) of a myosin IIA−/− immature DC migrating in a microchannel filled with 10 kDa AF647-Dextran. (a,b) Single macropinosomes were colour-coded to follow their intracellular transport. (c) Macropinosome velocity in WT, Ii−/− and myosin IIA−/− DCs. Bars show the medians (n=29 WT, 21 Ii−/− and 17 myosin IIA−/− from two independent experiments). A Kruskal–Wallis test was applied for statistical analysis. (d) Correlative graph of macropinosome retrograde speed versus myosin IIA patches intensity at the cell front (n=28 WT and 22 Ii−/− from two independent experiments). WT DCs correspond to Ii+/+ DCs (littermate controls). Scale bars, 5 μm.